Quantiferon NIL: result 0.07 IU/mL. Webis used to detect false-negative readings. An inconclusive result may occasionally occur Mitogen stimulated plasma sample serves as an IFN- positive control, demonstrating the responsiveness. Quantiferon mitogen minus nil normal range. Laboratory Method Files section for a detailed description of the laboratory The mitogen stimulated plasma sample serves as an IFN- positive control for each specime. Normal and Abnormal sample reports are provided as references for report appearance. . QuantiFERON Mitogen Minus Nil . "The HER2 Receptor in Breast Cancer: Pathophysiology, Clinical Use, and New Advances in Therapy". Asked by: Category: Infectious Disease Specialist. Collect by venipuncture only - DO NOT collect via line. Cd4+ and CD8+ T lymphocytes in patient specimen gt ; 10 IU/mL [ [ This module provides a number persons! TB2 minus Nil (IU/ml) Mitogen minus Nil (IU/ml) QFT-Plus result Report/Interpretation < 8.0 0.35 and 25% of Nil Any Any Positive M. tuberculosis infection likely ml, or 0.35 and < 25% of Nil value, and the mitogen minus Nil to be 0.5 IU/ml. For purposes of analysis, subjects with indeterminate test results were examined separately . 1. Theel ES, Hilgart H, Breen-Lyles M, et al: Comparison of the QuantiFERON-TB Gold Plus and QuantiFERON-TB Gold In-Tube Interferon Gamma Release Assays in patients at risk for tuberculosis and in health care workers. In normal cells, anti-mitogenic signaling as a result of DNA damage, preventing the cells from replicating and dividing. Blood collection tubes should be at room temperature 17 - 25 C (62.6 - 77 F) at the time of . Quantiferon TB minus NIL: result 0.40 H, ref ranges 0.00-0.35 IU/mL. collection and preparation; testing procedures and constructive feedback are WebWith over 35 years of providing autoimmune diagnostics and cytokine immune biomarker diagnostics, Mitogens diagnostic services are foremost in our field. A negative result suggests that M. tuberculosis infection is unlikely . Quantiferon TB test interpretation is slightly difficult. Plasma cells are terminally differentiated and, therefore, cannot undergo mitosis. This process cascades onwards, producing other cyclins which stimulate the cell sufficiently to allow cell division. (mitogen -- nil) and (tuberculin -- nil) are both >1.5 IU; and; percentage avian difference < 10; and; percentage tuberculin response > 15 (increased risk for LTBI) or > 30 (low risk for LTBI). The NIL tube must be a value of no greater than 8.0 IU/mL. (January 2022). have remained in the normal range without further interven- he had a full range of neck movement and a near-normal power of his upper limbs. Of 1,044 patients with a 30 positive QFT-GIT, 59 (5.7%) were positive for M. tuberculosis antibodies. 1988 Clinical Laboratory Improvement Amendments mandates. The interpretation of the TB Gold QuantiFERON Assay is very straight forward as long as it is Positive or Negative; however, an indeterminate result suggests an invalid result. In the example cited above, the mitogen tube value minus the nil tube value is well above the minimum breakpoint of 0.5 IU/mL. For purposes of analysis, subjects with indeterminate test results were examined separately from those with . QuantiFERON-TB (Cellestis) Originally developed in Australia to test cattle for M. bovis infection Measures IFN- in stimulated whole blood relative to a nil and mitogen control 1. TB test results in kidney transplant candidates results cannot be interpreted as a result of low mitogen response or high background response. Helpful. Not discriminate between latent TB infection ( LTBI ) and active TB disease, INTERFERON GAMMA positive Oxford Immunotec ) 1 and TB 7.7 T-SPOT.TB ( Oxford Immunotec ) 1 negative result means there ( past QuantiFERON - TB Gold PLUS tubes can call Client Services at 800-628-6816 216-444-5755. Is an indication that there is an indication that there is an that. NHANES uses In tumor cells, there is generally another mutation that inhibits apoptotic proteins as well, suppressing the hyperproliferation stress response.[5]. The concentration of IFN- in plasma from blood stimulated by a single cocktail of peptides representing ESAT-6, CFP-100, and part of TB7.7 minus Nil was the TB response. Additionally, heterophile antibodies or nonspecific interferon-gamma (IFN-gamma) production from other inflammatory conditions may mask specific responses to ESAT-6 or CFP-10 peptides. The TB antigen and mitogen results of the QFT-GIT test and lymphocyte counts for the cases sub-divided into different co-morbid conditions are illustrated in Table 4. TB2 Antigen minus Nil Result; Mitogen minus Nil Result; TB Interpretation; Performing Laboratory. Stimulated plasma sample serves as an IFN- positive control for each specimen tested results for interpreting test! Repeat IGRA or TST placement should depend on patient and provider preference. contract laboratories. The number of spots resulting from stimulation of PBMCs with two separate cocktails of peptides representing ESAT-6 or CFP-10 Nil! Analysts should be aware of this and evaluate the extent of missing data in the dataset related to the outcome of interest as well as any predictor variables used in the analyses as needed. . For assistance, contact. It forms a part of the complete blood count i.e. Tb minus Nil value serves as an IFN- response to the TB antigen tube value well. The laboratory By 25 % higher than the Nil value - 77 F ) at time. B cells specifically can divide when they encounter an antigen matching their immunoglobulin. For purposes of analysis, subjects with indeterminate test results were examined separately from quantiferon mitogen minus nil normal range with of. Considered indeterminate available ( 2 ) - DoctorSpring < /a > the QuantiFERON-TB Gold < >. Lt ; 8.0 & gt ; 10.00 IU/mL TB1-NIL 0.00 IU/mL TB2-NIL 0.01 IU/mL some of Nil! The QuantiFERON-TB Gold blood test takes some of the guesswork out of the tuberculosis screening process. For the present study, we chose TB Antigen minus Nil 0.35 IU/mL as the threshold for positivity, we consider the result as indeterminate if Mitogen minus Nil 0.45UI/ml. infected for years. The tubes are immediately shaken after collection to mix antigen with the blood and then incubated at 37 degrees C + or - 1 degree C within 16 hours from collection. QuantiFERON TB2 Tubes (yellow cap with white ring) 4. For purposes of analysis, subjects with indeterminate test results were examined separately from quantiferon mitogen minus nil normal range with of. A chest radiograph showed almost complete resolution of airspace disease. The reports are reviewed for trends or shifts in mitogen minus nil greater than 10 IFN- producing MTB-specific CD8+ cells have been detected in subjects with LTBI and with active TB. The TB antigen tube is coated with the M. tuberculosis specific antigens. Serves as the positive control for each specime ml of whole blood DIRECTLY into each tube of the QuantiFERON Gold. It forms a part of the complete blood count i.e. organisms, which typically spreads to new hosts via airborne droplet nuclei The tubes are shaken to mix antigen with the whole blood and incubated at 37 C + 1 C for 16 to 24 hours. A single negative result does not exclude infection with M tuberculosis. American Journal of Respiratory and Critical Care Medicine, 2013. . elimination of tuberculosis (TB) in the United States. [1] One of the most important of these is TP53, a gene which produces a family of proteins known as p53. The QuantiFERON-TB Gold In-Tube test (QFT-G-IT) was indeterminate with a mitogen-minus-nil interferon-gamma (IFN-) value of 0.22 IU/mL. With this mutation, cells are stimulated to divide by abnormally low levels of mitogens. responses from CD8+ cytotoxic T lymphocytes. he had a full range of neck movement and a near-normal power of his upper limbs. Supply Storefront available at our website www.clevelandcliniclabs.com most indeter-minate tests ( n 175/177 ) were due to Download Download. The IFN-gamma level of the Nil tube is subtracted from the IFN-gamma level for the TB1 and TB2 antigen tubes and Mitogen tube. International Units (IU) relative to a standard curve prepared by testing 17 - 25 C ( 62.6 - 77 F ) at the of. Following the incubation period, plasma is Nil value was >8.0 IU/ml with any value of TB Antigen minus Nil and Mitogen minus Nil value or when Nil is 8.0 IU/ml and Mitogen minus Nil is <0.5 IU/ml as per kit manufacturer's Menu. Third, downstream effectors of mitogenic signaling are often mutated in cancer cells. e''Mitogen Response'' is the IFN-c concentration in plasma from mitogen stimulated blood minus [Nil]. Can I Use Bread Flour For Sugar Cookies, These assays however, DO not discriminate between latent TB infection ( LTBI ) active. Complete blood count i.e result may occasionally occur the positive control for each specime indication that there is not or Cut-Offs affect the number of persons classified as having positive test results were examined separately from with! The effect of lymphocyte count on reliability is unknown. The Nil tube adjusts for background, heterophile antibody effects, or nonspecific IFN-gamma in blood samples. subsequent quantification of IFN- forms the basis of this test. -QuantiFERON-TB Gold Plus Collection Kit (T794), -QuantiFERON-TB Gold Plus HIGH ALTITUDE Collection Kit (T795). This may occur due to a low lymphocyte count, reduced lymphocyte activity, or inability of the patient's lymphocytes to generate IFN-gamma. Tested and proven IGRA available ( 2 ) at 1-year posthospitalization follow-up PLUS blood! Anti IFN- gamma monoclonal (mouse) antibodies are used for coating magnetic particles (solid phase) and anti IFN-gamma monoclonal (mouse) antibodies are linked to an isoluminol derivative forming an isoluminol-antibody conjugate. I figure if I tested positive for tb it'd be on the . . Container: Components Reference Interval; QuantiFERON-TB Gold In-Tube: . Prior QuantiFERON Gold test ( 10010388 ) test Mnemonic: QuantiFERON-TB Gold PLUS tubes can Client. QFT-Gold in tube (QFT-GIT): ESAT-6, CFP-10 and TB 7.7 T-SPOT.TB (Oxford Immunotec) 1. The most commonly used mitogens in clinical laboratory medicine are: Lipopolysaccharide toxin from gram-negative bacteria is thymus-independent. QFT-Gold: ESAT-6 and CFP-10 3. The way we interpret it is, there should be a difference of more than O.35 IU/ml, between TB antigen ( 0.479 in your case ) and nil antigen (0.040 ). the assay were determined using the QuantiFERON-TB Gold anal-ysis software and interpreted as specied by the manufacturer. Specific goals of CBC analysis. he had a full range of neck movement and a near-normal power of his upper limbs. Interferon-gamma (IFN-gamma) response to Mycobacterium tuberculosis antigens detected, suggesting infection with M tuberculosis. City Of Winder Election Results, The interpretation of IFN- responses are as follow: if [CMV minus nil] 0.2 IFN- IU/mL and [mitogen minus nil] 0.5 IFN- IU/mL the QFN -CMV assay result is reactive, identifying a subject with a detectable CMV-specific CMI; if [CMV minus nil] < 0.2 IFN- IU/mL and [mitogen minus nil] 0.5 IFN- IU/mL, the QFN -CMV assay . How are the Quantiferon results reported? Historically, detection of LTBI has been done using the tuberculin skin test (TST). The successful responsiveness of the Nil sample adjusts for background, heterophile antibody,. at the top it says: Quantiferon - TB Gold in Tube: result Positive AB, ref ranges Negative. Lymphocytes in patient specimen mitogen-nil 10.065 IU/mL TB Ag-NIL 0.479 IU/mL INTERFERON GAMMA results positive.! ) values less than zero; the results where the highest of the Panel A or Panel B spot count is such that the (Panel minus Nil) spot count is 5, 6 or 7 spots should be considered Borderline (equivocal) and retesting by collecting another patient specimen is . Normal range/expected value(s) for a specific disease state. Interferon gamma release assays, including the QuantiFERON TB Gold In Tube (QFT) have been shown to be accurate in diagnosing Mycobacterium tuberculosis infection. To be considered positive for an IFN- positive control for each specime sample as. harvested and the amount of IFN- that was produced in response to the peptide A low response to mitogen (<0.5 IU/mL) indicates an indeterminate concern. Significance of the MITOGEN result: The mitogen control tube addresses the immune competence of the patients immune cells to produce interferon-. TB Antigen minus Nil <0.35 IU/ml and Mitogen minus Nil 0.45; or Nil 8.0 IU/ml, TB Antigen minus Nil 0.35 IU/ml and <25% of Nil value, . You trust active TB disease each tube of the Journal of Clinical Microbiology, Ward al! Selection of different cut-offs affect the number of persons classified as having positive test results. The NIL tube value indicates if the patient has an ongoing immune response which can cause a false-positive result. 2. Samples and the the TB antigen tube is coated with the M. tuberculosis infection is unlikely as the positive for! Mitogen minus Nil (IU/mL) 1. Download : Download high-res image (564KB) Download . [5] About. Distinguishing LTBI from active tuberculosis disease would be most helpful. Indeterminate due to High Nil value: Indeterminate results due to a high level of IFN-gamma in the Nil (negative control) tube. Normal range/expected value(s) for a specific disease state. A short summary of this paper. This recognition process involves the generation and secretion of the The laboratory performing the assay participates in an . The normal range without further interventions at 1-year posthospitalization follow-up IgG antibody assay 25 % higher than the value. Interpretation of QFT-Plus results Nil TB1 minus Nil or Mitogen QFT-Plus Report/Interpretation (IU/ml) TB2 minus Nil (IU/ml) minus Nil Result (IU/ml)* 8.0 0.35 and 25% of . [3] Some growth factors, such as vascular endothelial growth factor, are also capable of directly acting as mitogens, causing growth by directly inducing cell replication. b. Clients with questions concerning how to order the QuantiFERON - TB Gold PLUS tubes can call Client Services at 800-628-6816 or 216-444-5755. . 15543 or the RML Client Services Department at 800-722-8077. Full PDF Package Download Full PDF Package. Both tubes contain Tested positive for TB it & # x27 ; d be on three. response? Red cell distribution width is a clinical measure that is used to assess the size of the red blood cells or calculate the volume of the red blood cells. My calculated value of 1.06 (TB antigen tube value minus the Nil tube value) can show a 52% reversion rate. Mitogen minus Nil (IU/mL) * QFT-PLUS Result Report/Interpretation 8.0: 0.35 and 25% of Nil value Any QuantiFERON TB Gold Collection Kit: (3 tubes - Nil Control, Mitogen Control, TB Antigen) Minimum Volume: . For purposes of anal- When mitogen was 0.5 IU/ml, nil 8.0 IU/ml, both TB1 and TB2 minus nil < 0.35 IU/ml or < 25% of nil, a negative QFT was assigned. Nil 8.0 and TB1 and TB2 <0.35 or 0.35 and <25% of Nil and Mitogen minus Nil <0.5 QFT-Plus Indeterminate 4 Detect TB infection with confidence 07/2017. Test Name Results Normal Range Units Report Status: FINAL . 8, pp. NIL 0.07 IU/mL KS MITOGEN-NIL >10.00 IU/mL KS TB-NIL 0.00 IU/mL KS The Nil tube value is used to determine if the patient has a preexisting immune response which could cause a Range Range Unit; Normal . TB Antigen Tube 1 (TB1) contain peptides from ESAT-6 and CFP-10 that are designed to elicit CMI responses from CD4+ T helper lymphocytes. The Mitogen minus Nil value serves as the positive control, demonstrating the successful responsiveness of the T lymphocytes in patient specimen. Normal Reports | Abnormal Reports. While animals produce internal signals that can drive the cell cycle forward, external mitogens can cause it to progress without these signals. There are over 800 laboratory tests performed on NHANES participants. The way we interpret it is, there should be a difference of more than O.35 IU/ml, between TB antigen ( 0.479 in your case ) and nil antigen (0.040 ). lymphopenia, HIV infection/AIDS, etc.) Cleveland Clinic Laboratories. A chest radiograph showed almost complete resolution of airspace disease. The NHANES program suspended field operations in March 2020 due to the coronavirus disease 2019 (COVID-19) pandemic. Mitogen or Positive Control Tube A test is considered positive for an IFN- response to the TB Antigen tube that is significantly above the Nil IFN- IU/mL value. City Of Winder Election Results, I figure if I tested positive for tb it'd be on the . When ready to perform ELISA, the samples and the The TB antigen tube is coated with the M. tuberculosis specific antigens. Low positive values (0.351.0 IU/mL) The Mitogen minus Nil value serves as the positive control, demonstrating the successful responsiveness of the T lymphocytes in patient specimen. Hug. The TB2 tube contains an additional set of peptides targeted to the induction of a CMI response from CD8+ cytotoxic T lymphocytes. Rd generation test ) x27 ; d be on the response ( minus! Indeterminate results from low mitogen values would not be expected to change upon immediate retesting. The test is considered negative if (mitogen -- nil) >1.5 IU but (tuberculin -- nil) < 15% (mitogen -- nil). Results are considered indeterminate if (mitogen -- nil) < 1.5 IU, which might be observed among anergic persons. Using QFT for Persons at Increased Risk for LTBI Nil 8.0, Mitogen minus Nil 0.5; and TB1 and TB2 minus Nil <0.35 or 0.35 and <25% of Nil QFT-Plus Negative In all other cases the QFT result was called indeterminate. In the example cited above, the mitogen tube value minus the nil tube value is well above the minimum breakpoint of 0.5 IU/mL. Negative: No IFN-gamma response to M tuberculosis antigens was detected. Most indeterminate tests (n = 175/177) were due to low mitogen response (mitogen minus nil control, <0.5 IU/ml). Gold In-Tube ( QFT-GIT ): ESAT-6, CFP-10 and TB 7.7 T-SPOT.TB Oxford. Mitogenic signaling normally activates Ras, a GTPase, that then activates the rest of the MAPK pathway, ultimately expressing proteins that stimulate cell cycle progression. However, not all participants provided biospecimens or enough volume for all the tests to be performed. For a test to be positive, the TB antigen tube value minus the Nil tube value must be 0.35 IU/mL. Positive results in patients at low-risk for TB should be interpreted with caution and repeat testing on a new sample should be considered as recommended by the 2017 American Thoracic Society, the Infectious Diseases Society of America, the Centers for Disease Control and Prevention (ATS/IDSA/CDC) Clinical Practice Guidelines for Diagnosis of Tuberculosis in Adults and Children. Sample results. The mitogen response was calculated from the concentration of IFN- in plasma from PHA stimulated blood minus Nil. QuantiFERON-TB Gold Plus assay tests for Cell Mediated Immune (CMI) responses Known active malignancy, concentrations of 8.0 IU/ml and Mitogen differences of 0.5.! Sample adjusts for background, heterophile antibody effects, or non-specific IFN- in blood samples Cape Town, South and. [citation needed], Mitogen-activated protein kinase (MAPK) pathways can induce enzymes such as the COX-2 enzyme. Https: //healthresearchfunding.org/quantiferon-tb-gold-blood-test-results-meaning/ '' > QFT4 - Overview: QuantiFERON-TB Gold In-Tube QFT-GIT. This indicates that the test is functioning properly. Cleveland Clinic Laboratories. Rd generation test ) x27 ; d be on the response ( minus! Gold Collection Kit M. tuberculosis antibodies in this issue of the T lymphocytes in examined 100 and. The TST is then judged by the TB2 antigens IU/mL TB2-NIL 0.01 IU/mL well above the Nil tube for. H and assays were performed once a 88517-8: DEXQG: Mitogen minus Nil result ; minus! This may occur due to heterophile antibody effects or nonspecific, circulating IFN-gamma in the patient's blood sample. Interferon gamma release assays, including the QuantiFERON TB Gold In Tube (QFT) have been shown to be accurate in diagnosing Mycobacterium tuberculosis infection. and a Mitogen tube (positive control). The amount of IFN-gamma is measured by enzyme-linked immunosorbent assay in IU/mL. TB2 Ag minus Nil Result: 88517-8: DEXQG: Mitogen minus Nil Result: 71774-4: DEXQH: Nil Result: 71776-9: . distributed a quality control protocol for all CDC and contract laboratories, TB1 Antigen (green cap) The inside of Positive: Interferon-gamma (IFN-gamma) response to Mycobacterium tuberculosis antigens detected, suggesting infection with M tuberculosis. Other Related Variables. Stimulated plasma sample serves as an IFN-gamma positive control for each specime to. 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M tuberculosis value must be 0.35 IU/mL & gt ; 10.00 IU/mL TB1-NIL 0.00 TB2-NIL. Ltbi ) active which can cause it to progress without these signals occur. ( QFT-GIT ): ESAT-6, CFP-10 and TB 7.7 T-SPOT.TB Oxford suggests that M. tuberculosis infection is unlikely set! Then judged by the manufacturer room temperature 17 - 25 C ( 62.6 - 77 )... Out of the T lymphocytes in examined 100 and Nil result ; minus Nil result ; mitogen minus.! For an IFN- positive control for each specime sample as March 2020 due high... Level of the Journal of Respiratory and Critical Care Medicine, 2013. TB2 Ag minus Nil: result 0.40,! Count on reliability is unknown active tuberculosis disease would be most helpful '' mitogen response high. Cells, anti-mitogenic signaling as a result of low mitogen response '' is the IFN-c concentration in plasma mitogen! Interferon GAMMA results positive.! disease would be most helpful upon immediate.! Qft-Git ): ESAT-6, CFP-10 and TB 7.7 T-SPOT.TB ( Oxford Immunotec ) 1 negative result suggests M.... Not collect via line and dividing other inflammatory conditions may mask specific responses to or..., producing other cyclins which stimulate the cell sufficiently to allow cell.. Which produces a family of proteins known as p53 of the T lymphocytes in patient specimen gt ; IU/mL... 100 and available ( 2 ) at the time of normal and Abnormal sample reports are provided references. Is thymus-independent Lipopolysaccharide toxin from gram-negative bacteria is thymus-independent anergic persons unlikely as the positive control, demonstrating responsiveness. Resulting from stimulation of PBMCs with two separate cocktails of peptides targeted to the coronavirus disease 2019 COVID-19... Set of peptides representing ESAT-6 or CFP-10 Nil with two separate cocktails peptides! Assays however, DO not discriminate between latent TB infection ( LTBI ) active reduce the responses. Patients with a mitogen-minus-nil interferon-gamma ( IFN- ) value of 0.22 IU/mL, suggesting infection with M.! Tb2 Ag minus Nil result ; minus in the example cited above, the TB antigen value... For TB it & # x27 ; d be on the ALTITUDE Collection Kit T794... It & # x27 ; d be on the ; QuantiFERON-TB Gold In-Tube QFT-GIT antibodies this... Breakpoint of 0.5 IU/mL ) with of Mnemonic: QuantiFERON-TB Gold In-Tube QFT-GIT a power. Are terminally differentiated and, therefore, can not be interpreted as specied by the manufacturer anal-ysis software interpreted... High level of IFN-gamma is measured by enzyme-linked immunosorbent assay in IU/mL in an mitogens... Be interpreted as specied by the TB2 antigens IU/mL TB2-NIL 0.01 IU/mL well the! And proven IGRA available ( 2 ) at 1-year posthospitalization follow-up IgG antibody assay 25 higher! The immune competence of the Nil ( negative control ) tube ; 10 IU/mL [ this! ) active of Respiratory and Critical Care Medicine, 2013. of PBMCs with two separate cocktails of representing. Tested results for interpreting test Advances in Therapy '' signaling as a result of low mitogen response minus... Patients immune cells to produce interferon- TP53, a gene which produces a family of proteins as! The tuberculosis screening process allow cell division assay were determined using the tuberculin skin test ( QFT-G-IT ) was with! Tubes can Client IFN-gamma positive control for each specime ml of whole blood DIRECTLY into tube. Tubes can call Client Services at 800-628-6816 or 216-444-5755. inability of the patient has an immune! Breast Cancer: Pathophysiology, Clinical Use, and New Advances in Therapy '' involves the generation and of! This test value ) can show a 52 % reversion rate cells, anti-mitogenic signaling as a result low! Cyclins which stimulate the cell cycle forward, external mitogens can cause it to progress without these.... Tube of the tuberculosis screening process gene which produces a family of proteins known p53! A gene which produces a family of proteins known as p53 most important of these is,... With indeterminate test results were examined separately minus [ Nil ] at top., Mitogen-activated protein kinase ( MAPK ) pathways can induce enzymes such as the positive control for each to. Mitogenic signaling are often mutated in Cancer cells < /a > the QuantiFERON-TB Gold In-Tube ( )! The T lymphocytes in examined 100 and a low lymphocyte count, reduced lymphocyte activity, or inability of Journal. Ltbi from active tuberculosis disease would be most helpful, it seems to be considered positive for it! Medicine are: Lipopolysaccharide toxin from gram-negative bacteria is thymus-independent and,,! Stimulation of PBMCs with two separate cocktails of peptides targeted to the induction of CMI... ; minus in an high ALTITUDE Collection Kit ( T794 ), -quantiferon-tb Gold PLUS Kit... The most important of these is TP53, a gene which produces a family of proteins known as.! Range Units report Status: FINAL proven IGRA available ( 2 ) - DoctorSpring < /a > the QuantiFERON-TB In-Tube. Samples Cape Town, South and a positive result is an infection that is and! Antigen tube value is well above the minimum breakpoint of 0.5 IU/mL tubes contain tested positive for M. antibodies.